论文部分内容阅读
目的 :选择单羟酚类物质酪氨酸为标准物 ,建立测定尿中单羟酚类物质的实验方法。方法 :采用可见分光光度和正交实验 ,确定测定酪氨酸含量体系的各项最优化条件 ,并运用于测定各类正常人及肿瘤患者尿液中单羟酚类物质的含量。结果 :在一定浓度范围内酪氨酸含量与吸光度呈现良好的线性关系 ,运用该法测定各类正常人和肿瘤患者尿液单羟酚类物质时 ,其敏感度为 78.1% ,特异性为 96 .0 % ,阳性预测值为 85 .4 % ,阴性预测值为 93.4 %。结论 :此法可作为肿瘤患者辅助诊断的指标 ,是一种无创、快速、低廉的人群肿瘤筛查方法。
OBJECTIVE: To select monohydroxyphenolic tyrosine as standard and establish an experimental method for the determination of monohydric phenols in urine. Methods: By using visible spectrophotometry and orthogonal experiments, the optimal conditions for determination of tyrosine content were determined and used to determine the content of monohydroxyphenols in urine of all kinds of normal people and cancer patients. Results: There was a good linear relationship between tyrosine content and absorbance in a certain range of concentration. When this method was used to determine urinary monohydroxyphenols in all kinds of normal and cancer patients, the sensitivity and specificity were 78.1% and 96% respectively .0%, the positive predictive value was 85.4%, the negative predictive value was 93.4%. Conclusion: This method can be used as an indicator of the diagnosis of cancer patients, is a non-invasive, rapid and inexpensive method of cancer screening.