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甜菜是我国主要糖料作物之一,为从蛋白质组学水平了解甜菜对干旱胁迫的应答,进一步明确甜菜的抗旱分子机理,通过双向电泳技术对甜菜耐旱品种HI0466正常供水和干旱胁迫条件下叶片差异表达蛋白质组进行了对比研究,质谱鉴定共获得了17个差异表达蛋白点,包括丰度上调的10个,新出现的蛋白点6个,丰度下调的1个。功能分类分析表明干旱胁迫蛋白在光合作用、物质与能量代谢、活性氧清除、细胞结构、物质运输和蛋白质合成等方面发挥作用。
Beet is one of the main sugar crops in China. In order to understand the response of sugar beet to drought stress from the level of proteomics, the molecular mechanism of drought resistance of sugar beet is further clarified. By using two-dimensional electrophoresis, the sugar content of leaves of sugar beet drought tolerant variety HI0466 under normal water supply and drought stress A total of 17 differentially expressed protein spots were identified by mass spectrometry, including 10 up-regulated, 6 newly emerged proteins and 1 down-regulated abundance. Functional classification analysis showed that drought stress proteins play roles in photosynthesis, metabolism of matter and energy, scavenging of active oxygen, cell structure, transport of materials and protein synthesis.