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目的 观察TGF β1对体外培养人前列腺上皮细胞增殖与凋亡的调节作用。 方法 建立原代人前列腺上皮细胞株 13例 ,角化蛋白、PSA等免疫组织化学染色鉴定培养细胞 ,绘制细胞生长曲线、运用BrdU方法和细胞凋亡原位染色观察传代后细胞生长状况及不同剂量TGF β1对细胞增殖与凋亡的作用。 结果 培养细胞角化蛋白、PSA染色阳性。TGF β1抑制细胞增殖并诱导细胞凋亡 ,作用与剂量成正比 ,EGF与TGF β1相互减弱对方的作用。 结论 TGF与TGF β1对前列腺上皮细胞增殖与凋亡状态起重要的调节作用。
Objective To observe the regulatory effect of TGFβ1 on the proliferation and apoptosis of human prostatic epithelial cells in vitro. Methods Thirteen cases of primary human prostate epithelial cell line were established. The cultured cells were identified by immunohistochemical staining such as keratin protein and PSA. The cell growth curve was drawn. BrdU staining and in situ cell apoptosis were used to observe the cell growth status and the cell proliferation in different doses Effect of TGF β1 on Cell Proliferation and Apoptosis. Results Cultured keratinocytes were positive for PSA staining. TGF-β1 inhibits cell proliferation and induces apoptosis, which is proportional to the dose. EGF and TGFβ1 weaken each other’s role. Conclusion TGF and TGFβ1 play an important role in the regulation of proliferation and apoptosis of prostatic epithelial cells.