目的评估两种冷冻方法对小鼠卵母细胞和单精子卵胞浆注射(ICSI)胚胎发育潜能的影响,以期为辅助生殖领域人卵母细胞的冷冻保存和胚胎发育潜能的研究提供实验依据。方法分别用玻璃化冷冻与程序化冷冻对小鼠MⅡ期的卵母细胞进行冷冻保存,解冻后存活的卵母细胞用于ICSI注射,然后将得到的胚胎体外发育并进行胚胎移植。用卡方和单因素的方差分析进行统计学分析,并比较两种方法冻存的卵母细胞解冻后的存活率,ICSI胚胎的2-细胞率、8-细胞率、囊胚率和妊娠率等。结果采用玻璃化冷冻法冷冻的MⅡ期卵母细胞存活率为81.6%,明显高于程序冷冻法组的70.7%。两组得到的胚胎在2-细胞形成率方面没有差异,但是8-细胞率和囊胚发育率差异均有统计学意义。两组假孕母体的妊娠率(63.6%和50.0%)和幼崽出生率(20.0%和12.4%)均没有统计学差异。结论与程序化冷冻相比,玻璃化冷冻对于小鼠MⅡ期卵母细胞的保存效果较好。解冻后卵母细胞用于ICSI,玻璃化冷冻组的胚胎得到较高的8-细胞率和囊胚发育率。但这两组的囊胚移植得到的妊娠率和出生率没有差异。 Objective To evaluate the effects of two freezing methods on embryonic developmental potential of mouse oocytes and spermatogenic cytoplasmic injections (ICSI) in order to provide experimental evidences for the study of cryopreservation and embryonic development potential of human oocytes in assisted reproductive field. Methods Mice oocytes were cryopreserved by vitrification and programmed freezing, respectively. After thawing, the surviving oocytes were used for ICSI injection. The resulting embryos were in vitro developed and embryo transfer was performed. Statistical analysis was conducted by chi-square test and one-way analysis of variance (ANOVA). The survival rates of frozen-thawed oocytes after thawing were compared. The 2-cell rate, 8-cell rate, blastocyst rate and pregnancy rate Wait. Results The vitality of M Ⅱ oocytes frozen by vitrification was 81.6%, which was significantly higher than 70.7% of the frozen group. There was no difference in the rate of 2-cell formation between the two groups of embryos, but there was significant difference between 8-cell rate and blastocyst rate. Pregnancy rates (63.6% and 50.0%) and pup birth rates (20.0% and 12.4%) between the two groups were not statistically different. Conclusion Compared with programmed freezing, vitrification is better for the preservation of mouse M Ⅱ oocytes. After thawing, oocytes were used for ICSI. The vitrified embryos obtained higher 8-cell and blastocyst rates. However, there was no difference in pregnancy rates and birth rates between the two groups of blastocysts.