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采用反相微乳液法制备了壳聚糖-二氧化硅复合纳米粒子,通过透射电镜（TEM）、红外光谱方法、Zeta电位实验等对所合成的复合纳米粒子的结构和性质等进行了表征.结果显示,基于壳聚糖与silica之间超分子作用所形成的复合纳米粒子呈规则的球形,粒径为50 nm左右,具有良好的单分散性和微孔结构,Zeta电位测定结果表明复合纳米粒子具有正的ζ（Zeta）电位值.基于复合纳米粒子的微孔结构和显正电性,以小牛胸腺DNA为对象研究了复合纳米粒子对DNA的吸附特性.研究结果表明在pH=6的磷酸盐（PBS）缓冲中,复合纳米粒子不但呈现出对小牛胸腺DNA良好的吸附能力,而且还由于壳聚糖与SiO2纳米材料的复合而展现出优良的抗酶切割能力和细胞亲和性.据此发现,我们发展了一种有望用于向细胞内转载DNA的新材料.“,”A novel mesoporous chitosan-silica nanoparticles was synthesized with the water-in-oil（W/O） reverse microemulsion method.By characterizing this nanoparticles with transmission electron microscopy,IR spectra and the zeta potential experiments,it was found that those nanoparticles were spherical and uni-form with 50 nm size and presented the mesoporous structure.Based on these findings,the adsorption ability of this nanoparticles for calf thymus DNA was studied.The relating results showed that this nanoparticles had a stronger adsorption ability to DNA in PBS buffer（pH=6）,they also presented the better biocompati-bility for cell and could be used for protect DNA from Dnase I cleavage.