论文部分内容阅读
目的对采用流式磁珠PCR-SSOP法复核2例HLA-A位点罕见基因型,查找分析原因。方法对2 688例HLA-A、B、DRB1SBT基因分型结果中43例罕见型结果,采用流式磁珠PCR-SSOP法复核,查找差异,确定分型,并分析差异结果原因。结果 2例罕见基因型在流式磁珠PCR-SSOP法同一批号03A高分试剂中均有1组磁珠假阳性,造成HLA-A位点罕见基因型漏检。2例罕见基因型在流式磁珠PCR-SSOP法同一批号004高分试剂检测结果与SBT结果一致。结论 HLA罕见基因型不能轻易排除,应用多种方法复核,保证HLA基因分型结果的准确。
Objective To investigate the rare genotypes of two cases of HLA-A loci by flow cytometry and PCR-SSOP. Methods Forty-three cases of genotyping HLA-A, B and DRB1SBT genotypes were analyzed by flow cytometry (PCR-SSOP). The differences were identified and the genotypes were determined. The causes of the differences were analyzed. Results Two rare genotypes had a set of magnetic beads false positive in the same batch number of 03A by flow cytometry PCR-SSOP, which caused a rare genotype of HLA-A locus. 2 cases of rare genotypes in the flow-bead PCR-SSOP method with the same lot number 004 high-grade test results and SBT results. Conclusion HLA rare genotypes can not be easily ruled out, and multiple methods should be used to check the accuracy of HLA genotyping results.