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为探讨纤维支气管镜直视下毛刷脱落细胞中Ｈ－ｒａｓ基因突变在肺癌中的诊断价值，采用在纤维支气管镜毛刷脱落细胞中应用ＰＣＲ－ＲＦＩＰ方法（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ－ｒｅｓｔｒｉｃｔｉｏｎｆｒａｇｍｅｎｔｌｅｎｇｔｈｐｏｌｙｍｏｒｐｈｉｓｍｓ）检测Ｈ－ｒａｓ基因，人突变。对５０例肺癌及２０例肺部炎症患者支气管上皮脱落细胞进行了检测，结果肺癌中Ｎ－ｒａｓ基因突变为５４％（２７／５０）．鳞癌为５９．１％（１３／２２）．腺癌为５３．８％（７／１３），小细胞肺癌为３３．３％（２／６），肺透明细胞癌１００％（２／２），未能分型的肺癌４２．９％（３／７），肺炎组１０％（２／２０）。肺癌组的Ｈ－ｒａｓ基因突变明显高于肺炎组，二者有明显的差异（Ｐ＜０．０１）。研究肺癌Ｈ－ｒａｓ基因突变有较高的阳性，在肺癌与非肺癌组中有明显的差别，有希望成为肺癌诊断标记物。
To investigate the diagnostic value of H-ras gene mutations in exfoliated cells of brush under bronchoscope in lung cancer, PCR-RFIP (polymerase chain reaction-restriction fragment length polymorphisms) was used to detect H-ras gene in fibroblast brush-out cells. Mutations. The bronchial epithelial exfoliated cells were detected in 50 patients with lung cancer and 20 patients with pulmonary inflammation. The results showed that the N-ras mutation in lung cancer was 54% (27/50). Squamous cell carcinoma is 59.1% (13/22). The adenocarcinoma was 53.8% (7/13), the small cell lung cancer was 33.3% (2/6), the clear cell lung cancer was 100% (2/2), and the undifferentiated lung cancer was 42.9% ( 3/7), 10% (2/20) of pneumonia. The H-ras gene mutation in lung cancer group was significantly higher than that in pneumonia group. There was a significant difference between the two groups (P<0.01). The study of H-ras mutations in lung cancer is highly positive and there is a clear difference between lung cancer and non-cancer lung cancers. It is hopeful to become a diagnostic marker for lung cancer.