目的　了解卡氏肺孢子虫在HepG 2 (人肝癌细胞 )等 8种细胞系的增殖状况 ,寻找适合卡氏肺孢子虫 (Pc)增殖的支持细胞系。方法　分别以 5× 10 5个虫体接种于 8种细胞系上。另采用HepG 2细胞 ,分别接种不同数量的Pc ,观察 7d ,比较Pc在不同细胞及不同接种数量的增殖情况。 结果　在HepG 2细胞上Pc滋养体可增殖 7倍左右 ,增殖高峰大多在第 4、5天。接种 5× 10 5个Pc ,包囊和滋养体均可较好增殖。结论　HepG 2细胞是较好的卡氏肺孢子虫体外培养支持细胞系 ,接种量以每孔 5× 10 5个Pc包囊为宜 Objective To investigate the proliferation status of Pneumocystis carinii in HepG 2 (human hepatoma cell) and other 8 cell lines and to find a suitable cell line suitable for the proliferation of Pneumocystis carinii (Pc). Methods 5 × 10 5 parasites were inoculated on 8 cell lines respectively. The other HepG 2 cells were inoculated with different amounts of Pc, observed 7d, Pc in different cells and different inoculum size proliferation. The results of HepG 2 cells on the trophozoites can proliferate about 7 times the proliferation peak mostly in the first 4,5 days. Inoculation of 5 × 10 5 Pc, cysts and trophozoites can be better proliferation. Conclusions HepG 2 cells are the best supportive cell line for culturing Pneumocystis carinii in vitro with inoculation amount of 5 × 10 5 Pc per well