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60年以前,Wolbach已注意到缺乏维生素A的小鼠会发生癌前性上皮病变,但能被维生素A族物质(retinoid)逆转。体外实验表明,维生素A的衍生物——维甲酸(Retinoic Acid,RA)能抑制化学致癌物诱导的细胞恶变。随后又发现,RA能诱导人类早幼粒细胞性白血病细胞株HL-60的分化和成熟。这些工作引起了人们对RA的广泛兴趣和研究。本文就RA对人类正常造血细胞和白血病细胞的作用及其临床应用作一综述。一、RA对正常造血细胞集落生长的刺激作用迄今为止,RA对造血细胞的作用多半是在体外实验体系中观察的。许多作者证明RA能促进正常入骨髓细胞集落的生长。Douer等应用琼脂培养基培养正常人CFU-
Before 60 years, Wolbach had noticed that mice lacking vitamin A could develop precancerous epithelial lesions but could be reversed by retinoids. In vitro experiments showed that Retinoid Acid (RA), a derivative of vitamin A, can inhibit chemical carcinogen-induced cell malignant transformation. Subsequently, it was found that RA can induce the differentiation and maturation of human promyelocytic leukemia cell line HL-60. These work have led to people’s wide interest and research in RA. This article reviews the effects of RA on human normal hematopoietic and leukemia cells and their clinical applications. I. The stimulatory effect of RA on colony growth of normal hematopoietic cells So far, the effect of RA on hematopoietic cells has mostly been observed in in vitro experimental systems. Many authors have demonstrated that RA promotes the growth of normal bone marrow cell colonies. Douer et al. applied agar medium to culture normal human CFU-