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为证实我们的假设,即维甲酸物质不但能导致胎鼠颅面部的畸形,而且还能造成胸颈部内分泌腺(胸腺、甲状腺和甲状旁腺)的异常,这些器官的形成都是与神经脊细胞的移入有关的。于孕期第10天给母鼠125mg/kg全反型维甲酸(All-fran-retinoic acid),对照组给予相应量的载体溶液。分娩前24小时剖腹取出胎鼠,10％福尔马林浸泡固定10天,双管手术显微镜下解剖,检查颅、面部,心脏,大血管,胸腺,并对甲状腺,甲状旁腺进行组织学研究。对照组10只胎鼠未发现畸形,而实验组20只胎鼠中,有17只(85％)出现颅面部畸形,14只(70％)有心血管畸形,而胸腺、甲状腺、甲状旁腺和甲状腺畸形分别有14只(70％)、15只(75％)和7只(35％)。结果表明,维甲酸能导致胎鼠中鳃弓衍化而成并需神经脊参与的器官组织结构异常。
To confirm our hypothesis that retinoids not only cause deformities in the craniofacial area of the fetal rat, but also cause abnormalities in the endocrine glands (thymus, thyroid and parathyroid) in the thoracic and cervical organs, all of which are associated with neural crest Cell migration related. On day 10 of pregnancy, 125 mg / kg All-franretinoic acid was given to the female rats, and the control group was given the corresponding amount of carrier solution. Twenty-four hours before labor, fetuses were removed by cesarean section and immersed in 10% formalin for 10 days. Tissues were dissected under a double-tube surgical microscope to examine the tissues of the skull, face, heart, blood vessels, thymus, and thyroid and parathyroid glands . Ten fetuses in the control group showed no deformities, whereas 17 of 20 fetuses in the experimental group had craniofacial deformities and 14 (70%) had cardiovascular malformations, whereas thymus, thyroid, parathyroid and Thyroid abnormalities were 14 (70%), 15 (75%) and 7 (35%), respectively. The results show that retinoic acid can lead to fetal rat ephedra derived from the armpit and the need to participate in the neural crest organ tissue structure abnormalities.