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用不同浓度的东亚钳蝎素的多肽提取物PESV(4￣20μg/ml)作用于人脐静脉内皮细胞(HUVEC),观察HUVEC增殖活性和凋亡变化,增殖活性检测采用BrdU掺入的ELISA法,凋亡水平和凋亡相关基因Bcl-2和Bax表达的检测采用流式细胞术检测;用鸡胚尿囊膜(CAM)显示PESV对血管生成的抑制作用。结果显示,PESV抑制HUVEC的增殖,而对乳腺癌细胞MDA-MB-231的增殖无明显影响;PESV作用72h后,HUVEC凋亡相关基因Bcl-2表达降低,Bax表达增加,凋亡细胞比例增至10.5%,明显高于对照组;0.5mgPESV能明显抑制CAM新生血管的形成。因此,PESV具有良好的体外抗肿瘤血管生成活性,PESV作为一种肿瘤血管抑制剂的天然药物来源,其有效成分和药理作用有待进一步研究。
The proliferative activity and apoptosis of HUVECs were observed by using PESV (4-20μg / ml), a peptide extract of different concentrations of Asarum scorpionin, in human umbilical vein endothelial cells (HUVECs). Proliferation assay was used to detect BrdU incorporation , The level of apoptosis and the expression of apoptosis related genes Bcl-2 and Bax were detected by flow cytometry. The inhibitory effect of PESV on angiogenesis was also demonstrated by using chick embryo allantoic membrane (CAM). The results showed that PESV inhibited the proliferation of HUVEC and had no effect on the proliferation of breast cancer cells MDA-MB-231. After 72 hours of PESV treatment, the expression of Bcl-2, Bax and the percentage of apoptotic cells increased To 10.5%, significantly higher than the control group; 0.5mgPESV can significantly inhibit the formation of CAM neovascularization. Therefore, PESV has good anti-angiogenic activity in vitro. PESV, as a natural drug source for tumor angiogenesis inhibitor, has to be further studied for its active principle and pharmacological action.