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目的探讨膀胱尿路上皮癌组织Krüppel样因子8(KLF8)以及高迁移率族蛋白A2(HMGA2)表达水平及其与预后的相关性。方法选取2012年1月—2013年10月在郑州大学第五附属医院就诊的74例膀胱尿路上皮癌患者的肿瘤组织作为研究组,另选取同期在本院行膀胱切开取石术或开放前列腺切除术患者23例的膀胱黏膜作为对照组。采用免疫组化方法检测两组标本组织中KLF8和HMGA2蛋白表达水平。采用Cox回归分析KLF8及HMGA2蛋白的表达水平与患者生存预后的关系。结果研究组KLF8和HMGA2表达水平均高于对照组,差异有统计学意义(t=3.872,P=0.002;t=4.593,P=0.001)。膀胱尿路上皮癌患者临床分期与KLF8和HMGA2表达水平均呈正相关,差异有统计学意义(rs=0.489、0.561,P<0.05)。患者均随访至2015年4月,67例患者在随访截止时存活,总生存率为90.5%。多元Cox回归分析结果显示,临床病理分期、KLF8和HMGA2表达水平是膀胱尿路上皮癌患者生存预后的影响因素(P<0.05)。结论膀胱尿路上皮癌组织KLF8及HMGA2蛋白的高水平表达是患者生存预后的危险因素。
Objective To investigate the expression of Krüppel-like factor 8 (KLF8) and high mobility group box A2 (HMGA2) in bladder urothelial carcinoma and its correlation with prognosis. Methods Totally 74 patients with bladder urothelial carcinoma who were treated in the Fifth Affiliated Hospital of Zhengzhou University from January 2012 to October 2013 were selected as the study group. The patients underwent either cystectomy or open prostate in the same period. 23 cases of resection of the bladder mucosa as a control group. Immunohistochemistry was used to detect the expression of KLF8 and HMGA2 protein in two groups of specimens. Cox regression analysis KLF8 and HMGA2 protein expression and prognosis of patients with the relationship. Results The expression levels of KLF8 and HMGA2 in the study group were significantly higher than those in the control group (t = 3.872, P = 0.002; t = 4.593, P = 0.001). The clinical stage of bladder urothelial carcinoma was positively correlated with the expression of KLF8 and HMGA2, the difference was statistically significant (rs = 0.489, 0.561, P <0.05). All patients were followed up until April 2015, and 67 patients survived the follow-up. The overall survival rate was 90.5%. Multivariate Cox regression analysis showed that the clinicopathological stage, KLF8 and HMGA2 expression levels were the prognostic factors in patients with bladder urothelial carcinoma (P <0.05). Conclusion The high expression of KLF8 and HMGA2 protein in bladder urothelial carcinoma is a risk factor for prognosis.