论文部分内容阅读
目的测定24 h内血液中皮质醇含量,确定正常人和肿瘤患者的日周期节律性。方法采用放射免疫法测定30名健康志愿者和57名恶性肿瘤患者每日8:00,14:00,20:00及次日2:00血液中皮质醇(CORT)浓度。结果采用多因素方差分析及G reenhouse-G e isser法校正的单因素方差分析(P<0.01),表明组内各时间节点CORT浓度不完全相同,且CORT浓度存在时间依赖性。组间效应的方差分析结果(P<0.05),表明分组因素有意义,肿瘤组与对照组CORT浓度不完全相同。应用非参数统计方法(M ann-W h itney U,秩和检验)进行组间各时间节点的两两比较,结果显示两组间在8:00,14:00的CORT浓度存在明显差别。结论肿瘤组与对照组CORT浓度均随时间呈梯度和明暗交替变化,且肿瘤组的CORT浓度昼夜节律明显高于对照组。
Objective To determine the cortisol content in blood within 24 h and determine the rhythm of daily cycle in normal and cancer patients. Methods The concentration of cortisol (CORT) in blood was measured by radioimmunoassay in 8:00, 14:00, 20:00 and 2:00 in the next day in 30 healthy volunteers and 57 cancer patients. Results One-way analysis of variance (ANOVA) using the multivariate analysis of variance and the G reenhouse-G isser method (P<0.01) showed that the concentrations of CORT at different time points in the group were not exactly the same, and the CORT concentration was time-dependent. The analysis of variance between groups (P<0.05) showed that the grouping factor was significant, and the CORT concentrations in the tumor group and the control group were not exactly the same. Non-parametric statistical methods (Mann-Whitney U, rank sum test) were used to compare pairs of time points between groups. The results showed that there was a significant difference in CORT concentrations between the two groups at 8:00 and 14:00. Conclusion The CORT concentrations in the tumor group and the control group alternated with time and gradient, and the circadian rhythm of the CORT concentration in the tumor group was significantly higher than that in the control group.