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目的探讨胃癌中MUC1与β-catenin异常表达的意义及其与胃癌临床分期、组织学类型、肿瘤发生部位、肿瘤大小和核分裂指数等临床病理学关系。方法收集外科胃癌手术切除标本61例,应用HE染色切片进行组织病理学分类与核分裂指数评价,EnVision两步法行MUC1与β-catenin单克隆抗体免疫组织化学染色,10%以上肿瘤细胞出现胞浆聚集及细胞核着色被视为异常表达。结果本组胃癌中32例(52.46%)有MUC1异常表达,29例(47.54%)有β-catenin异常表达,MUC1与β-catenin异常表达一致率为73.77%。MUC1异常表达组核分裂指数增加(P=0.027),肿瘤在胃底贲门部相对好发(P=0.068)。MUC1或β-catenin单项异常表达与其它临床病理学指标无明显关系。MUC1与β-catenin共同异常表达时核分裂指数增高较MUC1单项异常表达以及MUC1与β-catenin无异常表达组更加明显(P=0.004)。结论β-catenin的异常表达表明部分胃癌出现Wnt/β-catenin信号通道异常活化。MUC1通过Wnt信号通道辅助活化因子作用促进β-catenin对胃癌细胞增殖的调控,两者共同异常表达明显促进胃癌细胞增殖;MUC1与β-catenin可作为反映胃癌生物学行为的有用标记。
Objective To investigate the significance of abnormal expression of MUC1 and β-catenin in gastric cancer and their clinicopathological relationship with clinical stage, histological type, tumor location, tumor size and mitotic index. Methods Totally 61 surgical specimens of surgical gastric cancer were collected. HE staining was used to evaluate the histopathological features and mitotic index. MUC1 and β-catenin monoclonal antibodies were immunohistochemically stained with EnVision in two steps. More than 10% of the tumor cells showed cytoplasm Aggregation and nuclear staining are considered aberrant. Results The abnormal expression of MUC1 was found in 32 cases (52.46%) in this group. The abnormal expression of β-catenin was found in 29 cases (47.54%). The coincident rate of abnormal expression of MUC1 and β-catenin was 73.77%. MUC1 abnormal expression increased mitotic index (P = 0.027), the tumor in the gastric cardia relatively good (P = 0.068). The single abnormal expression of MUC1 or β-catenin had no significant relationship with other clinicopathological parameters. Increased mitotic index of MUC1 andβ-catenin were more significant than single abnormal expression of MUC1 and no abnormal expression of MUC1 andβ-catenin (P = 0.004). Conclusion The abnormal expression of β-catenin indicates that Wnt / β-catenin signaling pathway is abnormally activated in some gastric cancers. MUC1 promotes the regulation of β-catenin on the proliferation of gastric cancer cells through the activation of Wnt signaling pathway. The abnormal expression of MUC1 can significantly promote the proliferation of gastric cancer cells. MUC1 and β-catenin may be useful markers to reflect the biological behavior of gastric cancer.