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目的分析Adper Prompt(AP)自酸蚀粘结剂对人牙龈成纤维细胞的毒性作用。方法用MTT法测定人牙龈成纤维细胞在Adper Prompt自酸蚀粘结剂不同浓度稀释液中培养4 d、7 d的吸光度(A)值,计算细胞相对增殖率(RGR),并结合流式细胞仪测定不同浓度稀释液中培养4 d后的人牙龈成纤维细胞不同增殖周期时相的DNA百分含量,计算细胞增殖指数百分比,来评估AP的细胞毒性。结果随作用时间和浓度的改变,Adper Prompt对细胞的毒性发生变化。时间越长,浓度越大,细胞相对增殖率(RGR)越小,细胞增殖指数百分比越小。结论Adper Prompt自酸蚀粘结剂在体外对人牙龈成纤维细胞有一定程度的细胞毒性,在操作过程中应采取措施保护牙龈组织。
Objective To analyze the toxic effects of Adper Prompt (AP) self-etching adhesive on human gingival fibroblasts. Methods The absorbance (A) of human gingival fibroblasts cultured in different concentrations of Adper Prompt self-etching adhesive for 4 d and 7 d were measured by MTT method. The relative cell proliferation rate (RGR) Cytotoxicity of AP was evaluated by measuring the DNA percentage of human gingival fibroblasts at different proliferation cycles after 4 days of culture in different dilutions and calculating the percentage of cell proliferation index. Results With the change of action time and concentration, the toxicity of Adper Prompt changed. The longer the concentration, the greater the relative cell proliferation rate (RGR), the smaller the percentage of cell proliferation index. Conclusions Adper Prompt self-etching adhesives have a degree of cytotoxicity on human gingival fibroblasts in vitro and should be taken to protect gingival tissues during operation.