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为了观察脑源性神经营养因子的特异性受体—酪氨酸激酶受体 B在发育过程中的大鼠海马 CA1和 CA3区的分布 ,本研究使用免疫组织化学 A BC法研究了生后几个时间段的酪氨酸激酶受体 B的分布特点。结果表明 :其免疫阳性产物仅出现在神经元胞体中且只位于胞浆 ,胶质细胞中未见分布。生后零天组偶见免疫阳性细胞 ;生后 5天组免疫阳性细胞较生后 0天组明显增多 ,但分布也尚较少 ;生后 10、15、2 0、30天 4组免疫阳性细胞呈明显的逐渐增多趋势。成年组的免疫阳性细胞与生后 30天组无明显差别。本实验结果提示 :发育早期大鼠海马 CA1和 CA 3区锥体细胞产生酪氨酸激酶受体 B,并通过对它的分泌调节 ,控制脑源性神经营养因子对神经元的作用 (突触的发生、发育、维持及神经元损伤后修复等
In order to observe the distribution of hippocampal CA1 and CA3 regions in rat hippocampus during the development of specific receptor of brain-derived neurotrophic factor-tyrosine kinase receptor B, we used immunohistochemical ABC method to study Distribution of tyrosine kinase receptor B over time. The results showed that the immunopositive products only appeared in the cytoplasm of neurons and only in the cytoplasm. No distribution was found in the glial cells. The number of immunopositive cells in the zero-day group after birth was significantly higher than that in the control group at day 5 post-immunization Cells showed a clear trend of gradual increase. There was no significant difference between the immunopositive cells in adult group and that in 30 days after birth. Our results suggest that tyrosine kinase receptor B is produced by pyramidal cells in the hippocampal CA1 and CA3 regions of rats in early developmental stages and that it controls the neuronal activity of brain-derived neurotrophic factors (synapses The occurrence, development, maintenance and repair of neuronal damage