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目的：探讨细胞凋亡与急性肺损伤（ＡＬＩ）发生、发展的关系以及Ｆａｓ和／ＦａｓＬ系统表达改变的意义。方法：复制内毒素性大鼠ＡＬＩ模型；采用ＴＵＮＥＬ法、原位杂交、半定量逆转录聚合酶链反应（ＳｑＲＴ ＰＣＲ）及免疫组化等技术观察大鼠ＡＬＩ发生过程中，肺组织细胞凋亡变化以及Ｆａｓ／ＦａｓＬ系统蛋白质和ｍ ＲＮＡ表达的改变。结果：内毒素性ＡＬＩ早期（＜ ２４ 小时），大鼠肺泡上皮细胞和肺血管内皮细胞凋亡明显增加；肺组织Ｆａｓ／ＦａｓＬｍ ＲＮＡ和蛋白质表达明显上调，且与肺组织细胞凋亡的增加相一致。结论：肺组织细胞凋亡以及Ｆａｓ／ＦａｓＬ系统表达明显上调可能参与大鼠ＡＬＩ的发病机制。
Objective: To investigate the relationship between apoptosis and the occurrence and development of acute lung injury (ALI) and the significance of the changes of Fas and / FasL system expression. Methods: The ALI model of endotoxin-induced rats was duplicated. Apoptosis of lung tissue was detected by TUNEL, in situ hybridization, SqRT PCR and immunohistochemistry. Changes and changes of Fas / FasL system protein and m RNA expression. Results: Apoptosis of rat alveolar epithelial cells and pulmonary vascular endothelial cells was significantly increased in early (<24 h) ALI group. The expression of Fas / FasLmRNA and protein in lung tissue was significantly up-regulated Consistent. Conclusion: Apoptosis of lung tissue and the upregulation of Fas / FasL system may be involved in the pathogenesis of ALI in rats.