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在临床开展骨原发性恶性肿瘤含瘤骨段体内灭活的基础上，对本法的可行性进行了研究。传统的体外灭活法的离体是为了切断经皮质骨的血液供给。如能证明本法同样可以切断血供，即可达到安全使用的目的。动物实验和解剖学研究证实，体内灭活法完全可以阻断血流，达到与体外灭活相同的结果。而不离体较离体灭活有明显的优点。
The feasibility of this method was studied based on the in vivo inactivation of tumor-bearing bone segments of primary malignant bone tumors. The traditional in vitro inactivation method is ex vivo to cut off the blood supply of cortical bone. If it can be proved that this law can also cut off blood supply, it can achieve the purpose of safe use. Animal experiments and anatomy studies have confirmed that in vivo inactivation can completely block blood flow and achieve the same results as in vitro inactivation. Not in vitro than in vitro inactivation has obvious advantages.