为了观察ｂｃｌ２反义ＲＮＡ对胃癌细胞ＳＧＣ７９０１的促凋亡作用及ＭＴＩＩ启动子的启动诱导活性，用分子克隆方法构建载体，以电泳术、电镜术、流式细胞术、原位杂交、裸鼠肿瘤抑制试验及化学发光分析等方法观察，结果表明，可诱导载体ｐＭＴｂＮ具有良好的诱导启动转录活性；Ｂｃｌ２蛋白的表达下降使胃癌细胞ＳＧＣ７９０１更易发生凋亡，致瘤性也发生改变 In order to observe the apoptosis-inducing effect of bcl-2 antisense RNA on gastric cancer cell SGC7901 and the start-inducing activity of the MT-II promoter, a vector was constructed by molecular cloning and electrophoresis, electron microscopy, flow cytometry, and in situ hybridization were performed. , nude mice tumor inhibition assay and chemiluminescence analysis and other methods observed, the results showed that the inducible vector pMTbN has good induction of transcriptional activity; Bcl 2 protein expression decreased gastric cancer cells SGC7901 more prone to apoptosis, tumorigenicity also occurs change