AIM:To determine the location of c-jun protein,dynamic changes in c-jun mRNA and protein expression,and ultrastructure characteristics in the rd mouse retina,following a single dose of brain-derived neurotrophic factor(BDNF) in a short period of time.· METHODS:A single intravitreal injection of BDNF at two dosages(25μg/L or 50μg/L) was given to the right eye of the rd mouse at age 2 and 3 weeks respectively.Two weeks after injection,the location of c-jun protein in the retina was observed by immunofluorescence detection,c-jun mRNA and protein expression in retinas were detected by quantitative real time polymerase chain reaction(RT-PCR) and western immunoblotting analysis,ultrastructure characteristics of retinas were detected by transmission electron microscope(TEM) observation.· RESULTS:c-jun protein was expressed in the inner nuclear layer(INL) of retina.BDNF at two dosages(25μg/L and 50μg/L) increased c-jun mRNA expression at PN-4 weeks respectively(P1 =0.019,P2=0.021).50μg/L BDNF increased c-jun protein expression at PN-4 weeks(P =0.000).The retinal ultrastructure was improved.· CONCLUSION:The effects of BDNF exerts on the c-jun expression in the retina are dose-dependent and time-dependent,which may mediate photoreceptor rescue indirectly in the pathological process of retinitis pigmentosa(RP)at early stage. AIM: To determine the location of c-jun protein, dynamic changes in c-jun mRNA and protein expression, and ultrastructure characteristics in the rd mouse retina, following a single dose of brain-derived neurotrophic factor (BDNF) in a short period of time. · METHODS: A single intravitreal injection of BDNF at two dosages (25 μg / L or 50 μg / L) was given to the right eye of the rd mouse at age 2 and 3 weeks. Two weeks after injection, the location of c -jun protein in the retina was observed by immunofluorescence detection, c-jun mRNA and protein expression in retinas were detected by quantitative real time polymerase chain reaction (RT-PCR) and western immunoblotting analysis, ultrastructure characteristics of retinas were detected by transmission electron microscope RESULTS: The c-jun protein was expressed in the inner nuclear layer (INL) of retina. BDNF at two dosages (25 μg / L and 50 μg / L) (P1 = 0.019, P2 = 0.021) .50 μg / CONCLUSION: The effects of BDNF exerts on the c-jun expression in the retina are dose-dependent and time-dependent , which may mediate photoreceptor rescue rescue in the pathological process of retinitis pigmentosa (RP) at early stage.