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本研究以1 535 bp的COI基因全长作为分子标记,通过测序得到橘小实蝇[Bactrocera dorsalis(Hendel)]的海南、广东、广西、湖南、湖北、云南、四川、江西、福建、浙江、上海及江苏的12个种群共240头橘小实蝇个体的COI基因,对其进行种群遗传结构研究,分析江苏无锡地区橘小实蝇的入侵来源。结果表明,研究所涉及的橘小实蝇种群间呈现较显著的遗传结构,但并未出现距离隔离现象,且随着采样点经度和纬度的增加单倍型多样性呈减弱趋势。12个橘小实蝇地理种群分为3个组群进行AMOVA分析显示,组群间变异占总体变异的1.46%,组群内种群间变异占总体变异的4.07%,组群内个体间变异占总体变异的94.47%。表明本研究所涉及范围内的橘小实蝇有由南向北扩散的趋势,无锡地区的橘小实蝇种群很可能来源于广东、江西及浙江地区。
In this study, the full-length COI gene of 1 535 bp was used as a molecular marker and sequenced to obtain the nucleotide sequence of Bactrocera dorsalis (Hendel) from Hainan, Guangdong, Guangxi, Hunan, Hubei, Yunnan, Sichuan, Jiangxi, Fujian, Zhejiang, COI genes from 240 populations of 12 individuals from Shanghai, Jiangsu and Jiangsu Provinces were collected and their genetic structure was studied. The origin of the invasion of the fruit fly from Wuxi, Jiangsu Province was analyzed. The results showed that there was a significant genetic structure among the populations involved in the study but no segregation of distance was observed. Haplotype diversity showed a decreasing trend with the increase of longitude and latitude. The AMOVA analysis of 12 populations showed that the variation among the groups accounted for 1.46% of the total variation, the intra-group variation accounted for 4.07% of the total variation, and the intra-group variation accounted for 94.47% of the total variation. The results showed that there was a trend of spreading from south to north in the scope of this research. The population of Trifolium pratense in Wuxi probably came from Guangdong, Jiangxi and Zhejiang provinces.