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研究Ca2+.CaM是否参与小麦抗叶锈病反应的过程,为更深入了解小麦抗叶锈病的反应机制奠定基础。采用离体培养的方法测定小麦种子经CaM拮抗剂TFP(三氟拉嗪)、CPZ(氯丙嗪)和W-7(N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide)以及CaCl2预处理后小麦叶片接种叶锈菌后在不同时间点的酶活性。另外用实时定量PCR的方法检测小麦接种亲和小种和非亲和小种后CaM不同亚型基因的表达情况。试验结果表明:小麦叶片接种非亲和性叶锈菌后,TFP、CPZ和W-7浸种处理抑制了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升;小麦叶片接种亲和性叶锈菌后,CaCl2预处理加剧了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升。这说明Ca2+.CaM信使系统可能在小麦抗叶锈病过程中起着重要作用,并且不同的CaM亚型可能调控不同的抗病途径。
The study of whether Ca2 + .CaM participates in the process of leaf rust resistance in wheat lays the foundation for further understanding of the reaction mechanism of leaf rust resistance in wheat. Wheat seeds were treated with the CaM antagonists TFP (trifluoperazine), CPZ (chlorpromazine) and W-7 (N- (6-aminohexyl) -5-chloro-1-naphthalene sulfonamide) Enzymatic Activities of Wheat Leaves Pretreated at Different Time after Inoculation of Leaf Rust. In addition, real-time quantitative PCR method was used to detect the expression of different subtypes of CaM in wheat-inoculated races and non-racial races. The results showed that the soaking of TFP, CPZ and W-7 inhibited the activities of peroxidase (POD), alanine aminotransferase (PAL), polyphenol oxidase (PPO) and catalase (CAT) activities. After inoculation of wheat leaves with the resistant leaf rust, CaCl2 pretreatment accelerated the activities of peroxidase (POD), alanine ammonia lyase (PAL) Increased oxidase (PPO) and catalase (CAT) activities. This suggests that the Ca2 + .CaM messenger system may play an important role in the leaf rust resistance of wheat and that different CaM subtypes may regulate different pathogenicity.