论文部分内容阅读
目的探讨肛门及肛管尖锐湿疣(CA)组织中人乳头瘤病毒(HPV)感染的基因型别及其临床意义。方法从287例肛门及肛管CA石蜡组织标本中提取23种HPV-DNA,采用基因扩增结合基因芯片技术进行基因型别检测,并分析临床病理资料。结果 CA组织标本中检出HPV阳性者209例,HPV感染率为72.82%(209/287)。其中一重HPV感染154例,阳性检出率为53.66%(154/287);多重HPV感染55例,阳性检出率为19.16%(55/287)。一重HPV感染中HPV 6型73例,阳性检出率为25.44%(73/287);HPV 11型71例,阳性检出率为24.74%(71/287)。多重HPV感染中,HPV 6+11型25例,占多重感染的45.46%(25/55);HPV 6+18型和HPV 6+11+16型各3例,各占多重感染的5.46%(3/55)。结论 HPV 6型、11型、6+11型、6+18型和6+11+16型感染是肛门及肛管CA的主要致病类型。基因扩增结合基因芯片技术是一种比较适合临床开展HPV分型检测的诊断方法,其敏感性高、特异性好,尤其适合HPV感染的分子流行病学研究。
Objective To investigate genotypes of human papillomavirus (HPV) infection in the anus and anal genital warts (CA) and its clinical significance. Methods Twenty-three types of HPV-DNA were extracted from 287 specimens of anal and anal CA paraffin tissues. Genotypes were detected by gene amplification combined with gene chip technique, and clinical and pathological data were analyzed. Results Among the CA tissue samples, 209 were positive for HPV, and the HPV infection rate was 72.82% (209/287). One case of HPV infection in 154 cases, the positive detection rate was 53.66% (154/287); 55 cases of multiple HPV infection, the positive detection rate was 19.16% (55/287). In a case of HPV infection, 73 cases were positive for HPV type 6, the positive rate was 25.44% (73/287). In 71 cases of HPV type 11, the positive rate was 24.74% (71/287). In multiple HPV infection, 25 cases were HPV type 6 + 11, accounting for 45.46% (25/55) of multiple infections; 3 cases were HPV type 6 + 18 and HPV type 6 + 11 + 16, accounting for 5.46% 3/55). Conclusions HPV type 6, type 11, type 6 + 11, type 6 + 18 and type 6 + 11 + 16 are the major causal agents for anal and anal CA. Gene amplification combined with gene chip technology is a more suitable for clinical diagnosis of HPV typing detection method, its high sensitivity and specificity, especially for HPV infection in molecular epidemiology.