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目的:探讨宫颈癌染色体3p14区域D3S1300、D3S1600位点微卫星不稳定性(MSI)及等位基因杂合性缺失(LOH)与HPV感染的关系。方法:选择3p14区域两个微卫星多态性位点,对41例宫颈癌石蜡切片中的正常组织和肿瘤组织,经PCR扩增及聚丙烯酰胺凝胶电泳和银染色,进行LOH及MSI检测,并采用HC-Ⅱ法检测患者高危型HPV感染情况。结果:41例样本中有25例至少存在1个位点的LOH,有8例发生MSI,频率相对较低。D3S1300、D3S160O位点LOH的发生率与临床分期、病理分级相关性有显著意义(P<0.05),而MSI与之无显著差异(P>0.05)。D3S1300、D3S1600位点LOH和MSI的发生率与HPV感染均无显著差异(P>0.05)。结论:3p14区域内D3S1300、D3S1600位点具有较高的LOH,提示这两个微卫星位点附近可能存在尚未克隆的与宫颈癌发生、发展相关的肿瘤抑制基因,其杂合性缺失和微卫星不稳定性与HPV感染均无明显相关,是独立于HPV感染之外的发病因素。
Objective: To investigate the relationship between microsatellite instability (MSI) and allelic loss of heterozygosity (LOH) at D3S1300 and D3S1600 loci on chromosome 3p14 in cervical cancer and HPV infection. Methods: Two microsatellite loci in 3p14 region were selected. The normal tissues and tumor tissues of 41 cases of cervical cancer paraffin sections were selected and subjected to LOH and MSI detection by PCR amplification, polyacrylamide gel electrophoresis and silver staining. , And using HC-Ⅱ method to detect high-risk HPV infection in patients. Results: There were 25 cases of LOH in at least one locus in 41 cases, MSI in 8 cases and relatively low frequency. The incidence of LOH in D3S1300 and D3S160O sites was significantly correlated with clinical stage and pathological grade (P <0.05), but not with MSI (P> 0.05). There was no significant difference in the incidence of LOH and MSI between D3S1300 and D3S1600 sites and HPV infection (P> 0.05). CONCLUSION: D3S1300 and D3S1600 sites in 3p14 region have higher LOH, suggesting that there may exist tumor suppressor genes that have not been cloned in the vicinity of the two microsatellite loci, and their loss of heterozygosity and microsatellite Instability and HPV infection were not significantly correlated, is independent of HPV infection outside the risk factors.