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大肠杆菌表达系统在红霉素基因工程中的应用面临着许多难题 :聚酮合成酶中ACP的后修饰、6_dEB生物合成前体供应、大环内酯糖基化修饰等。通过向大肠杆菌中转入枯草杆菌磷酸泛酰巯基转移酶基因sfp ,使大肠杆菌中合成的ACP得以后修饰 ;6_dEB生物合成前体丙酰CoA和丙二酰CoA可通过Pfeifer途径和Dayem途径提供 ;目前 ,在大肠杆菌体内已可以合成 6_dEB。利用大肠杆菌系统合成新的大环内酯化合物、对大环内酯糖基化修饰和提高大肠杆菌系统合成大环内酯类抗生素产量将是今后研究的重点。
E. coli expression system in erythromycin gene engineering applications are faced with many problems: polyketide synthase ACP post-modification, 6_dEB biosynthesis precursor supply, macrolide glycosylation modification. The ACP synthesized in E. coli was post-modified by the transfer of the Bacillus subtilis phosphopantetheinyl transferase gene sfp into E. coli; the propanoyl-CoA and malonyl-CoA precursors for 6-dEB biosynthesis were available via the Pfeifer pathway and the Dayem pathway At present, 6_dEB can be synthesized in E. coli. The use of E. coli system synthesis of new macrolide compounds, macrolide glycosylation modification and improve the production of macrolide antibiotics in E. coli system will be the focus of future research.