论文部分内容阅读
目的:通过体外实验评价紫杉醇衍生物GT对人喉癌细胞系Hep-2的抑制作用。方法:应用MTT法测定GT对Hep-2细胞的增殖抑制作用;通过细胞形态学及流式细胞术考察GT对Hep-2细胞诱导凋亡的作用。结果:MTT实验显示GT对Hep-2细胞具有明显的增殖抑制作用;当GT作用细胞后,在光镜下能观察到较为典型的细胞凋亡的形态学变化,如细胞核固缩、染色质凝集成新月型紧贴于核膜周边,核碎裂、染色质断片化、凋亡小体形成等。流式细胞仪分析结果可见G2/M期的细胞比例明显增加,并出现典型的亚二倍体“凋亡小峰”。结论:GT对Hep-2细胞的生长有明显的抑制作用,其效果与紫杉醇基本相同,可使细胞分裂阻滞于G2/M期,并诱导肿瘤细胞凋亡,有进一步研究开发的价值。
OBJECTIVE: To evaluate the inhibitory effect of paclitaxel derivative GT on human laryngeal carcinoma cell line Hep-2 in vitro. Methods: The inhibitory effect of GT on the proliferation of Hep-2 cells was determined by MTT assay. The effect of GT on the apoptosis of Hep-2 cells was investigated by cell morphology and flow cytometry. Results: MTT assay showed that GT could significantly inhibit the proliferation of Hep-2 cells. When GT cells were treated, morphological changes of apoptotic cells such as nuclear condensation, chromatin condensation Into the crescent-type close to the nuclear envelope around the nuclear fragmentation, chromatin fragmentation, apoptotic body formation. Flow cytometry analysis showed that the proportion of cells in G2 / M phase increased significantly, and the typical sub-diploid “apoptotic peak” appeared. CONCLUSION: GT can significantly inhibit the growth of Hep-2 cells. Its effect is similar to that of paclitaxel. It can block cell division in G2 / M phase and induce apoptosis of tumor cells, which has the value of further research and development.