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用 10 0个 RAPD引物分析了具有广泛遗传资源代表性和较大应用面积的 2 4个水稻雄性不育系、 3个保持系和 3个生产中应用最广的恢复系。其中 72个引物具有多态性。从中筛选出 14个 RAPD引物 ,可检测出重演性较好的多态性片段 4 7带 ,能够有效地区分所有供试的雄性不育系及恢复系。杂交水稻亲本的聚类分析结果表明 :( 1)我国水稻雄性不育系遗传资源比较丰富 ,但生产中主要应用的不育系遗传背景单一。 ( 2 )在籼稻内部 ,不育系的遗传变异较恢复系少。 ( 3)应用面积较大的恢复系与雄性不育系分别聚类于不同的类群 ,且遗传关系较远。
A total of 240 rice male sterile lines, 3 maintainer lines and 3 restorer lines which were the most widely used in the production were analyzed with 10 0 RAPD primers with broad genetic resource representation and large application area. 72 of them have polymorphism. A total of 14 RAPD primers were screened out to detect polymorphic fragments with good reproducibility, which could effectively distinguish all tested male sterile lines and restorer lines. The results of cluster analysis of hybrid rice parents showed that: (1) The genetic resources of male sterile lines in our country are rich, but the main genetic background of sterile lines used in production is single. (2) In indica rice, the genetic variation of sterile lines is less than that of restorer lines. (3) The restorer lines and male sterile lines with larger application area are clustered in different taxa, respectively, and the genetic relationship is far.