论文部分内容阅读
目的研究丝甘蛋白聚糖(serglycin)的表达高低是否会影响乳腺癌细胞系对多柔比星的敏感性。方法用瞬时干扰的方法敲低丝甘蛋白聚糖在MDA-MB-231中的表达水平,且用qRT-PCR,免疫荧光的方法验证敲低效率;用MTS法测定多柔比星在阴性对照(NC)组和Si-丝甘蛋白聚糖(Si-SG)组中的IC_(50)值,以及测定在0.018μmol·L-1多柔比星的作用下两组的增殖曲线;用克隆形成的方法观察在不同药物压力下两组克隆形成数的差异。结果瞬时干扰的效率达到70%以上;MTS方法检测Si-SG组的IC_(50)值以及药物压力下的增殖曲线均显著低于NC组;且在不同药物压力下的克隆形成能力也显著低于NC组。结论在MDA-MB-231中瞬时干扰丝甘蛋白聚糖能够显著增强MDA-MB-231对多柔比星的敏感性。
Objective To investigate whether the expression of serglycin affects the sensitivity of breast cancer cell lines to doxorubicin. Methods The instantaneous interference method was used to knock out the expression level of glycan in MDA-MB-231, and the knockdown efficiency was verified by qRT-PCR and immunofluorescence. The MTS assay was used to detect the expression of doxorubicin in the negative control IC50 value in the group of NC and Si-SG, and the proliferation curve of the two groups under the action of 0.018 μmol·L-1 doxorubicin; The formation of the method observed under different drug pressure differences between the two groups of clone formation. Results The efficiency of transient interference was more than 70%. The IC 50 value of Si-SG group and the proliferation curve under drug pressure were significantly lower than those of NC group by MTS method. The clonogenic capacity under different drug pressure was also significantly lower In the NC group. Conclusion The transient interference of glycan with MDA-MB-231 can significantly enhance the sensitivity of MDA-MB-231 to doxorubicin.