为探索细胞黏附分子和细胞通讯的相关变化与癌恶性表型的关系，用免疫印迹、免疫荧光及荧光染料传输方法对转化的人胃细胞和３株人胃癌细胞系的Ｅ－ｃａｄｈｅｒｉｎ，Ｎ－ｃａｄｈｅｒｉｎ，α－ｃａｔｅｎｉｎ，β－ｃａｔｅｎｉｎ和细胞通讯进行研究．哺乳类正常胃黏膜细胞只表达钙黏蛋白Ｅ－ｃａｄｈｅｒｉｎ亚型而不表达Ｎ－ｃａｄｈｅｒｉｎ，Ｅ－ｃａｄｈｅｒｉｎ免疫荧光分布在细胞膜上，与膜内面黏着斑蛋白α－ｃａｔｅｎｉｎ，β－ｃａｔｅｎｉｎ分布一致，表明有Ｅ－ｃａｄｈｅｒｉｎ／ ｃａｔｅｎｉｎ（α－，β－）复合体存在．转化胃细胞和胃癌细胞的 Ｅ－ｃａｄｈｅｒｉｎ抑制，却表达Ｎ－ｃａｄｈｅｒｉｎ，与α－ｃａｔｅｎｉｎ，β－ｃａｔｅｎｉｎ免疫荧光共定位，形成 Ｎ－ｃａｄｈｅｒｉｎ／ｃａｔｅｎｉｎ（α－，β－）复合体．免疫印迹与免疫荧光染色结果一致．正常胃黏膜和转化的胃细胞都表达细胞通讯蛋白Ｃｘ３２，分布在膜间隙连接，有通讯功能．胃癌细胞Ｃｘ３２表达抑制，通讯功能缺陷．以上提示细胞黏合与细胞通讯分别介导的信号途径在胃癌细胞内的改变是癌变的相关事件．其中钙黏蛋白亚型从Ｅ－ｃａｄｈｅｒｉｎ向Ｎ－ｃａｄｈｅｒｉｎ的变异以前未见报道． In order to explore the relationship between cell adhesion molecules and cell-associated changes and the malignant phenotype of cancer, Western blot, immunofluorescence, and fluorescent dye delivery methods were used to transform human gastric cancer cells and three human gastric cancer cell lines E-cadherin, N- Cadherin, α-catenin, β-catenin and cell communication were studied. Mammalian normal gastric mucosa cells only expressed cadherin E-cadherin isoform but not N-cadherin. E-cadherin immunofluorescence was distributed on the cell membrane, consistent with the distribution of α-catenin and β-catenin in the membrane. There are E-cadherin/catenin (α-,β-) complexes present. E-cadherin inhibited the transformation of gastric cells and gastric cancer cells, but expressed N-cadherin, colocalized with α-catenin, β-catenin immunofluorescence, and formed N-cadherin/catenin (α-, β-) complexes. Western blotting was consistent with immunofluorescence staining. Normal gastric mucosa and transformed gastric cells express cell communication protein Cx32, which is located in the gap junction of the membrane and has communication function. Suppression of Cx32 expression in gastric cancer cells and communication defects. The above suggested that the changes of the signal pathway mediated by cell adhesion and cell communication in gastric cancer cells are related to canceration. The cadherin subtypes from E-cadherin to N-cadherin have not been reported previously.