参考国内外已完成测序的庚型肝炎病毒（ＧＢＶＣ／ＨＧＶ）基因序列，选取毒株间系列较保守的基因片段，并合成与之互补的核苷酸序列（Ａｎｔｉｓｅｎｓｅ），用末端转移酶将荧光素Ｎ６ｄｄＡＴＰ标记该片段，制成庚肝病毒基因探针。在严格控制温度的条件下，与固定于硝酸纤维膜（ＮＣ膜）上血清斑点杂交，洗膜后与抗荧光素碱性磷酸酶（ＡＰ）结合，加底物后化学发光自显影判断结果。该方法检测与套式逆转录聚合酶链反应（ＮｅｓｔｅｄＲＴＰＣＲ）检测结果的阳性符合率为８８．２％，阴性符合率为１００％；并且与其他相关病毒基因无交叉反应，具有较好的特异性与灵敏性。其检测结果比ＥＩＡ法检测庚肝病毒抗体更具临床意义。 According to the sequence of gene of hepatitis C virus (GBVC / HGV) that has been sequenced at home and abroad, the more conserved gene segments of the strains were selected and their complementary nucleotide sequences (Antisense) were synthesized. This fragment was labeled with fluorescein-N6-ddATP to make G gene probe. Under the condition of strict temperature control, it was hybridized with serum spots immobilized on nitrocellulose membrane (NC membrane), and then washed with anti-fluorescein-alkaline phosphatase (AP) result. The positive coincidence rate of this method with that of Nested RTPCR was 88.2%, and the negative coincidence rate was 100%. There was no cross-reaction with other related virus genes, which was better The specificity and sensitivity. The test results than the EIA method to detect hepatic G virus antibodies more clinical significance.