论文部分内容阅读
为观察免疫吸附特异性清除循环肿瘤坏死因子－α（ＴＮＦ－α）对内毒素休克时氧自由基代谢的影响，给新西兰白兔一次性静注内毒素８．０×１０９ｃｆｕ／ｋｇ，免疫吸附组和空灌流组分别于１ｈ后经抗ＴＮＦ－α单克隆抗体亲和免疫吸附柱或未偶联抗体的空灌流柱进行血液灌流２ｈ，对照组不予血液灌流，观察血压、血浆ＴＮＦ－α活性和ＭＤＡ含量、全血ＧＳＨ－Ｐｘ活力的变化。结果发现免疫吸附治疗后，血浆ＴＮＦ－α水平迅速下降，低血压状态明显改善，血浆ＭＤＡ含量明显降低，全血ＧＳＨ－Ｐｘ活力显著增高。提示免疫吸附特异性清除循环ＴＮＦ－α能显著减少氧自由基的生成，抑制脂质过氧化作用。
To observe the effect of specific removal of circulating tumor necrosis factor-α(TNF-α) on the metabolism of oxygen free radicals during endotoxin shock, New Zealand white rabbits were intravenously injected with endotoxin (8.0×10 9 cfu/kg). After 1 hour, the rats in the group and the empty perfusion group were perfused with anti-TNF-α monoclonal antibody affinity immunoadsorption column or unconjugated antibody perfusion column for 2 hours. The control group was not perfused with blood, and blood pressure and plasma TNF-α were observed. Activity and MDA content, changes in whole blood GSH-Px activity. The results showed that after the immunoadsorption therapy, plasma TNF-α levels rapidly decreased, the state of hypotension was significantly improved, the plasma MDA content was significantly reduced, and the activity of whole blood GSH-Px was significantly increased. It is suggested that the specific removal of circulating TNF-α by immunosuppression can significantly reduce the production of oxygen free radicals and inhibit lipid peroxidation.