目的调查中俄边境黑瞎子岛地区蜱种构成及其携带病原体状况。方法于2013年5月间在黑瞎子岛地区采用人工小时布旗法拖蜱,种类鉴定后进行11种病原体检测。森林脑炎、新布尼亚病毒和克里米亚-刚果出血热采用RT-PCR方法检测;立克次体、贝氏柯克斯体、伯氏疏螺旋体、土拉杆菌、巴尔通体、埃立克体、无形体和巴贝西原虫均采用PCR方法检测。对阳性扩增产物测序,并利用MEGA 5.0软件与Gen Bank中国内外已知菌株基因序列进行比对和系统发育分析。结果共拖蜱165只,经鉴定隶属于1科3属4种,即硬蜱科(Ixodidae),硬蜱属(Ixodes),全沟硬蜱(I.persulcatus),革蜱属(Dermacentor),森林革蜱(D.silvarum),血蜱属(Haema physalis),嗜群血蜱(H.concinna)和日本血蜱(H.japonica),分别占总体构成24.85%、12.12%、23.03%和40.00%。伯氏疏螺旋体、土拉杆菌、巴尔通体、无形体、埃立克体、森林脑炎黄病毒属、克里米亚-刚果出血热和新布尼亚病毒阳性检出率均为0;立克次体阳性检出率为15.76%(26/165),贝氏柯克斯体阳性检出率为0.6%(1/165),巴贝西原虫阳性检出率为0.6%(1/165)。其中有1例全沟硬蜱检测出巴贝西原虫和立克次体复合感染。经统计学分析得出蜱种与立克次体的感染有统计学意义(χ2=84.1391,P<0.05),以森林革蜱的感染率最高。经MEGA 5.0软件进行系统发育分析,得知立克次体以斑点热群劳式立克次体为主。结论此次调查证明黑瞎子岛地区蜱种以日本血蜱为主,森林革蜱感染立克次体概率最高,全沟硬蜱存在复合感染现象,因此应着重加强黑瞎子岛地区蜱种监测与防控。 Objective To investigate the constitution of ticks and the status of pathogen-bearing ticks in Heishuizidao area on the border of China and Russia. Methods In May 2013, artificial ticks were used to pull ticks in Heishuizidao area. 11 species of pathogens were detected after the species was identified. Forest encephalitis, the New Bunyan virus and the Crimean-Congo haemorrhagic fever were detected by RT-PCR; Rickettsia, Bemisia, Borrelia, Bordetella, Bartonella, Rickettsia, Anaplasma and Babesi protozoa were detected by PCR method. The positive amplification products were sequenced and compared with the known gene sequences of Gen Bank in China and abroad by MEGA 5.0 software and phylogenetic analysis. Results Totally 165 ticks were collected and identified as belonging to 4 genera, 1 genera, 3 genera, namely Ixodidae, Ixodes, I. persulcatus, Dermacentor, D.silvarum, Haema physalis, H.concinna and H.japonica accounted for 24.85%, 12.12%, 23.03% and 40.00% of the total, respectively %. Borrelia burgdorferi, Bacillus terrestris, Bartonella, Anaplasma, Ehrlichia, Flaviviridae, Crimean-Congo haemorrhagic fever and New Bunyan virus were all 0; The positive rate of gram positive was 15.76% (26/165), that of Bemisia cocos positive was 0.6% (1/165), and the positive rate of B. babici was 0.6% (1/165 ). One case of Ictalurus punctatus detected a composite infection of Babesi protozoon and rickettsia. Statistical analysis showed that the infection rates of ticks and rickettsia were statistically significant (χ2 = 84.1391, P <0.05), with the highest infection rate of forest ticks. Phylogenetic analysis using MEGA 5.0 software revealed that Rickettsia was dominated by spotted fever group Rickettsia. Conclusion The investigation shows that the majority of ticks in Heishuizidao area are mainly Japanese blood-flies, and the highest probability of Rickettsia gracilis infection is Rickettsia black-headed Ixodes and the phenomenon of composite infection of Ixodes nigra. Therefore, Prevention and control.