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目的:探讨色氨酸羟化酶1(tryptophan hyroxylase 1,TPH1)基因多态性和色氨酸羟化酶2(tryptophan hyroxylase 2,TPH2)基因多态性与青年人自杀未遂行为的关系。方法:采用i MLDRTM多重SNP分型技术对102例青年自杀未遂者(自杀未遂组)和112名青年健康对照(对照组)TPH1基因多态性位点rs1800532、rs211105、rs4537731、rs684302、rs7933505和TPH2基因多态性位点rs10748185、rs11178997、rs1386493、rs1843809、rs4448731、rs7305115、rs9325202进行基因分型,并对基因型、等位基因、单体型频率进行比较。结果:(1)单位点分析显示:以上12个多态性位点基因型及等位基因频率分布在自杀未遂组与对照组之间差异无统计学意义(P>0.05);(2)单体型分析显示:由TPH1基因5个位点组成的单体型G-G-C-C-G、G-G-T-T-G、G-TC-C-G、G-T-T-C-G、T-T-T-T-A频率在自杀未遂组和对照组间差异无统计学意义(P>0.05);由TPH2基因7个位点组成的单体型G-T-G-T-T-A-G频率在自杀未遂组和对照组间差异有统计学意义(χ2=10.034,P=0.002),经Bonferroni校正检验后,差异仍有统计学意义(P=0.024)。结论:TPH2基因7个SNP位点构成的单体型G-T-G-T-T-A-G可能与增加青年人的自杀未遂相关。
Objective: To investigate the relationship between polymorphisms of tryptophan hyroxylase 1 (TPH1) and tryptophan hyroxylase 2 (TPH2) gene and attempted suicide in young adults. Methods: The polymorphisms of TPH1 gene rs1800532, rs211105, rs4537731, rs684302, rs7933505 and TPH2 in 102 young suicide attempt group (suicide attempt group) and 112 young healthy control group (control group) were detected by i MLDRTM multiple SNP typing. The genotypes of rs10748185, rs11178997, rs1386493, rs1843809, rs4448731, rs7305115 and rs9325202 were genotyped and the frequencies of genotype, allele and haplotype were compared. Results: (1) Single point analysis showed that there was no significant difference in genotype and allele frequency distribution between the above 12 polymorphic loci and the control group (P> 0.05); (2) Body size analysis showed that there was no significant difference in frequencies of haplotype GGCCG, GGTTG, GTCCG, GTTCG and TTTTA between the suicide group and the control group (P> 0.05). The frequency of haplotype GTGTTAG composed of 7 loci of TPH2 gene was significantly different between suicide attempted group and control group (χ2 = 10.034, P = 0.002). After Bonferroni test, the difference was still significant P = 0.024). CONCLUSION: The haplotype G-T-G-T-T-A-G consisting of seven SNP loci of TPH2 gene may be associated with increased suicide attempts in young people.