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目的 研究5种抗氧化剂对细胞膜流动性的防护作用。方法 用粘附式细胞仪(ACAS570) 检测。结果与结论 紫外线(UVB)照射能迅速增高NIH3T3细胞中的脂质过氧化物。UVB照射10 min的NIH3T3细胞 (阳性对照组) 膜流动性明显低于阴性对照组(正常NIH3T3细胞,不用UVB照射)。超氧化物歧化酶(SOD)的保护作用不明显,加入该抗氧化剂的样本其细胞膜的流动性降低到与阳性对照组相同的数量级。维生素E、过氧化氢酶(CAT)、SOD+CAT、茶多酚的保护作用较明显,加入这些抗氧化剂的样本其细胞膜的流动性与阳性对照组比较,均高出一个数量级。
Objective To study the protective effect of five kinds of antioxidants on cell membrane fluidity. Methods: Adherent cytometry (ACAS570) detection. Results and Conclusion Ultraviolet (UVB) irradiation rapidly increased lipid peroxides in NIH3T3 cells. The membrane fluidity of NIH3T3 cells (positive control group) exposed to UVB for 10 min was significantly lower than that of the negative control group (normal NIH3T3 cells without UVB irradiation). The protective effect of superoxide dismutase (SOD) was not obvious. The samples with this antioxidant were reduced in cell membrane fluidity to the same order of magnitude as the positive control. The protective effects of vitamin E, catalase (CAT), SOD + CAT and tea polyphenols were obvious. Compared with the positive control group, the samples containing these antioxidants had an order of magnitude higher mobility.