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目的:研究左、右归丸及其拆方对去卵巢大鼠肝脏LPL、PPAR-γ基因与蛋白表达的影响,从而在分子水平说明其影响脂质代谢的机制。方法:90只2月龄SD大鼠,随机分为正常组(Control),假手术组(SHAM),模型组(OVX),补佳乐组(BJL),左归丸组(ZGW),右归丸组(YGW),共同药组(GTY),滋阴药组(ZYY),补阳药组(BYY),每组10只,正常组不做处理,假手术组麻醉后开腹切除卵巢周围适量脂肪组织,其余各组常规切除双侧卵巢。手术后1周,分别以蒸馏水、蒸馏水、蒸馏水、阳性对照药戊酸雌二醇片混悬液、左归丸、右归丸、两方共同药、滋阴药、补阳药的水煎液给各组大鼠灌胃。12周后处死大鼠,取大鼠肝脏,用实时荧光定量PCR技术检测脂蛋白酯酶(LPL)、过氧化物酶体增殖物激活受体-γ(PPAR-γ)的mRNA表达,用免疫组化方法检测大鼠肝脏LPL、PPAR-γ的蛋白表达。结果:与模型组比较,左、右归丸及其拆方均能上调LPL、PPAR-γmRNA的表达水平(P<0.01),上调LPL、PPAR-γ蛋白的表达水平。结论:左、右归丸及其拆方均可上调肝脏中LPL、PPAR-γ基因与蛋白的表达水平,这可能是其调节脂质代谢的机制之一。
Objective: To study the effects of Zuogui Gui Wan and its decomposed formulas on LPL and PPAR-γ gene and protein expression in the livers of ovariectomized rats, and to elucidate the mechanism of its influence on lipid metabolism at the molecular level. Methods: Ninety two-month-old Sprague-Dawley rats were randomly divided into four groups: control group, sham operation group, OVX group, BJL group, ZGW group, The rats in the normal group were treated with YGW, GTY, ZYY and BYY. The ovariectomized rats in the sham operation group Around the right amount of adipose tissue, the remaining groups routine excision of both ovaries. One week after the operation, distilled water, distilled water, distilled water, positive control drug estradiol valerate tablets suspension, Zuo Gui Wan, yougui pill, both common drugs, nourishing drugs, yang medicine decoction Gavage to each group of rats. After 12 weeks, the rats were sacrificed and the livers of rats were sacrificed. The mRNA of lipoprotein lipase (LPL) and peroxisome proliferator activated receptor-γ (PPAR-γ) was detected by real-time fluorescence quantitative PCR. Histochemistry was used to detect the protein expression of LPL and PPAR-γ in rat liver. Results: Compared with the model group, Zuogui Gui Wan and its disassembled formulas could up-regulate the expression of LPL and PPAR-γ mRNA (P <0.01) and up-regulate the expression of LPL and PPAR-γ. CONCLUSION: Zuogui Gui Wan and its disassembled formulas can upregulate the expression of LPL and PPAR-γ gene and protein in the liver, which may be one of the mechanisms regulating lipid metabolism.