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目的探讨庚型肝炎感染患者是否存在双重感染和多重感染。方法应用庚型肝炎病毒（ＨＧＶ）ＮＳ３区逆转录聚合酶链反应技术检测了ＨＧＶ系列稀释的质控血清及ＡｂｂｏｔＧＢＶ－Ｃ参比样品中ＨＧＶＲＮＡ，并对９０例丙型肝炎病毒（ＨＣＶ）ＲＮＡ阳性和１２例乙型肝炎、丙型肝炎双重感染献血员进行了ＨＧＶＲＮＡ的检测。结果ＨＧＶ系列稀释质控血清１０－１～１０－５均为阳性；１０－６为阴性。２份ＡｂｂｏｔＧＢＶ－Ｃ样品均为ＨＧＶＲＮＡ阳性。９０例ＨＣＶＲＮＡ阳性样品中，８例ＨＧＶＲＮＡ阳性（１７．８％）；１２例乙、丙双重感染者中４例（４／１２）ＨＧＶＲＮＡ阳性。结论不仅存在ＨＣＶ及ＨＧＶ双重感染，也存在多重感染。
Objective To investigate whether there is double infection and multiple infection in patients with hepatitis G infection. Methods HGV RNA in HGV serially diluted control serum and AbbotGBV-C reference samples were detected by reverse transcription-polymerase chain reaction (RT-PCR) with hepatitis G virus (HGV) NS3 region and positive for 90 cases of hepatitis C virus (HCV) RNA And 12 cases of hepatitis B and C double infection blood donors were detected HGVRNA. Results HGV serial dilutions of control serum 10-1 ~ 10-5 were positive; 10-6 was negative. Two samples of AbbotGBV-C were positive for HGV RNA. Of the 90 HCV RNA positive samples, 8 were positive for HGVRNA (17.8%), and 4 (4/12) were positive for HGV RNA in 12 patients with B and C infection. Conclusion There are not only double infection of HCV and HGV, but also multiple infections.