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大多数化学致癌物均可影响DNA、RNA和蛋白质合成的过程。本文研究了从食管癌高发区林县粮食中分离出的互隔交链孢霉菌株261提取物261-B_2-3对这些大分子物质合成的影响。研究表明261-B_2-3对DNA和蛋白质的合成有明显的抑制,其作用强度呈明显的浓度相关性。但是它对RNA的合成无明显影响。三种大分子物质的合成对于261-B_2-3的敏感性顺序为:蛋白质>DNA>RNA。261-B_2-3对DNA和蛋白质抑制作用的类型和机理并不相同。在中止261-B_2-3的作用后,DNA的合成率持续下降,形成一谷值,属于模板损伤型,而蛋白质则呈递增型合成曲线,为干扰代谢型。261-B_2-3对大分子合成的干扰导致了细胞生物学和生化过程的紊乱,并有可能在诱变过程中起到一定作用。
Most chemical carcinogens can affect DNA, RNA and protein synthesis. In this paper, we studied the effects of 261-B_2-3, an extract from Alternaria alternata strain 261, isolated from Linxian County, a high incidence area of esophageal cancer, on the synthesis of these macromolecules. Studies have shown that 261-B_2-3 on DNA and protein synthesis was significantly inhibited, the intensity was significant concentration-dependent. However, it has no significant effect on RNA synthesis. The sensitivities of the three macromolecules to 261-B_2-3 were as follows: protein> DNA> RNA. 261-B_2-3 The types and mechanisms of DNA and protein inhibition are not the same. After the abolition of the role of 261-B_2-3, DNA synthesis rate continued to decline, forming a valley, belonging to the template damage, while the protein was an incremental synthetic curve, as interfering metabolisms. 261-B_2-3 Interference with macromolecular synthesis leads to disruption of cell biology and biochemical processes and may play a role in mutagenesis.