本文对动物肝脏中维生素Ａ的经典〔１〕的荧光测定法进行了改进。改进后的方法是把维生素Ａ的乙醚提取液在氮气流下挥发至干后，不经薄层展板分离，而直接将其干渣溶解于环己烷中，用荧光分光光度法进行检测。将两种方法比较后的结果表明，改进后的方法回收率高，重复性好，维生素Ａ在整个过程中损失小；且快速、方便。维生素Ａ的环己烷溶液激发波长为３４５ｎｍ，发射波长为４７６ｎｍ。 In this paper, animal liver in vitamin A classic [1] fluorimetry was improved. The improved method is that after the vitamin A ether extract is volatilized to dryness under a nitrogen stream, the dried residue is directly dissolved in cyclohexane without being separated by a thin plate, and detected by fluorescence spectrophotometry. The results of comparing the two methods show that the improved method has high recovery and good repeatability, and the loss of vitamin A in the whole process is small; and it is fast and convenient. The excitation wavelength of vitamin A cyclohexane solution was 345 nm and the emission wavelength was 476 nm.