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目的探讨慢性激怒应激对大鼠胃窦平滑肌动力及下丘脑和血浆促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、瘦素、胃动素的影响,以及柴胡疏肝散的干预作用。方法将40只Wistar大鼠随机分为4组,每组10只,正常组不给任何刺激;模型组、柴胡舒肝散组、生理盐水组均给予夹尾刺激,每次30min,每天2次,连续刺激14天。柴胡舒肝散组、生理盐水组每次应激后分别立即灌胃2ml柴胡散[27.5g/(kg.d)]及生理盐水,每天2次。14天后检测各组大鼠胃窦肌条收缩活动,下丘脑及血浆CRH、ACTH、瘦素和胃动素水平。结果与正常组比较,模型组大鼠离体胃窦肌条收缩频率和振幅明显减少(P<0.01),下丘脑CRH、瘦素及血浆CRH、ACTH、瘦素浓度均明显增高(P<0.01),而下丘脑与血浆胃动素浓度则显著下降(P<0.01)。与模型组比较,柴胡舒肝散组胃窦肌条收缩频率和振幅显著增加(P<0.01),下丘脑和血浆CRH、ACTH及瘦素浓度显著降低(P<0.01),而下丘脑和血浆胃动素浓度则明显上升(P<0.01)。结论柴胡舒肝散可增加慢性激怒应激大鼠胃窦平滑肌动力,改善脑肠肽水平。
Objective To investigate the effects of chronic irritable stress on the motility of smooth muscle in gastric antrum and the effects of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), leptin and motilin in hypothalamus and plasma, San’s intervention. Methods Forty Wistar rats were randomly divided into 4 groups (10 rats in each group). The rats in the normal group were given no stimulation. The rats in model group, Bupleurum Shugan Powder group and saline group Times, continuous stimulation 14 days. Bupleurum Shugan powder group, normal saline group were given 2ml Chaihu San [27.5g / (kg · d)] and saline twice a day after each stress. The contractile activity of antral myenteric muscle strips, the levels of CRH, ACTH, leptin and motilin in the hypothalamus and plasma were detected 14 days later. Results Compared with the normal group, the contraction frequency and amplitude of the isolated gastric strips in the model group were significantly decreased (P <0.01). The concentrations of CRH, leptin, plasma CRH, ACTH and leptin were significantly increased in the hypothalamus (P <0.01) ), While the concentrations of hypothalamus and plasma motilin decreased significantly (P <0.01). Compared with the model group, the frequency and amplitude of contraction of gastric antral strips in Bupleurum shuganan group were significantly increased (P <0.01), the concentrations of CRH, ACTH and leptin in hypothalamus and plasma were significantly decreased (P <0.01) Plasma motilin concentration was significantly increased (P <0.01). Conclusion Chaihu Shugan Powder can increase the dynamic of gastric smooth muscle in chronic irritated rats and improve the level of ghrelin.