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目的了解特发性血小板减少性紫癜(ITP)外周血单个核细胞(PBMCs)Toll样受体(TLRs)的mRNA表达情况。方法采用RT-PCR方法(SYBR GreenⅠ荧光染料结合法),对30名ITP患者和30名健康者的PBMCs中所表达的TLR2、4、6、7、9 mRNA进行相对定量检测。结果ITP组TLR2和TLR7的mRNA相对表达量均明显高于对照组(P<0.05),而TLR4、TLR6、TLR9的mRNA相对表达量两组间无明显差异(P>0.05)。结论TLR2和TLR7在ITP患者中表达水平上调,可能在ITP发病机制中起重要作用。
Objective To understand the mRNA expression of Toll-like receptors (TLRs) in idiopathic thrombocytopenic purpura (ITP) peripheral blood mononuclear cells (PBMCs). Methods The relative quantification of TLR2, 4, 6, 7 and 9 mRNA expression in PBMCs of 30 ITP patients and 30 healthy controls was detected by RT-PCR (SYBR Green Ⅰ fluorochrome binding assay). Results The mRNA expressions of TLR2 and TLR7 in ITP group were significantly higher than those in control group (P <0.05). There was no significant difference between the two groups in mRNA expression of TLR4, TLR6 and TLR9 (P> 0.05). Conclusion The upregulation of TLR2 and TLR7 in ITP patients may play an important role in the pathogenesis of ITP.