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目的:探讨转化生长因子TGFβ1T869C基因多态性及其血清水平与子宫内膜异位症的关系。方法:用聚合酶链反应扩增TGFβ1T869C基因片段,PstⅠ内切酶进行限制性酶切反应并经测序证实。结果:子宫内膜异位症组TGFβ1 T869 C/C、C/T、T/T表型频率分别为0.210 0、0.600 0、0.190 0,对照组分别为0.250 0、0.580 0、0.170 0;子宫内膜异位症组TGFβ1 T869C、TGFβ1 T869T基因频率分别为0.510 0、0.490 0。对照组分别为0.540 0、0.460 0,TGFβ1T869C各种基因型频率在内异症组与对照组之间的差异无统计学意义(P>0.05)。结论:TGFβ1T869C基因多态性与子宫内膜异位症无关联。
Objective: To investigate the relationship between transforming growth factor TGFβ1T869C polymorphism and serum levels of endometriosis. Methods: The gene fragment of TGFβ1T869C was amplified by polymerase chain reaction (PCR). The restriction endonucleases of PstⅠ endonuclease were digested with restriction endonuclease and confirmed by sequencing. Results: The frequencies of C / T, C / T and T / T of TGFβ1 T869 in endometriosis group were 0.210 0,0.600 0,0.190 0 respectively, while those in control group were 0.250 0,0.580 0,0.170 0 respectively. The frequencies of TGFβ1 T869C and TGFβ1 T869T in endometriosis group were 0.510 0 and 0.490 0 respectively. The control group were 0.540 0,0.460 0 respectively. The frequencies of TGFβ1T869C genotypes in endometriosis group were not significantly different from those in control group (P> 0.05). Conclusion: There is no correlation between TGFβ1T869C polymorphism and endometriosis.