根据水稻种子及其贮藏蛋白特性 ,结合普通实验室条件 ,对分离水稻种子全蛋白及其各蛋白组分的方法进行改进 ,建立了筛选水稻种子贮藏蛋白突变体的新方法。讨论了抽提水稻种子全蛋白时试剂加入的最佳方式以及各蛋白组分分级分离时的最佳条件。新方法简便易行 ,快速高效 ,重复性好。将该法用于种子资源库筛选 ,得到了较满意的结果。 According to the characteristics of rice seeds and their storage proteins, combined with common laboratory conditions, the method of separating total protein and its protein components of rice seeds was improved, and a new method of screening mutant rice seed storage proteins was established. The best way of reagent addition when extracting whole protein of rice seeds and the best conditions for fractionation of each protein component are discussed. The new method is simple, fast and efficient, reproducible. This method was applied to the screening of seed resources and got satisfactory results.