论文部分内容阅读
为评价脂多糖对妊娠17天孕鼠的前列腺素(PG)产物的产生和对催产素的反应性的影响,对妊娠17天Wistar鼠腹腔内注射消炎痛或生理盐水,1小时后再注射脂多糖或生理盐水。分4组,第1组(生理盐水组)两次均注射生理盐水,第2组(消炎痛组)先注射消炎痛,再注射生理盐水,第3组(脂多糖组)先注射生理盐水,再注射脂多糖,第4组(消炎痛-脂多糖组)先注射消炎痛,再注射脂多糖。3小时后用乙醚麻醉孕鼠,无菌下开腹,从下腔静脉取血分离血清,切除子宫,从子宫角分离每个羊膜囊,收集羊水,分别测定血清和羊水中内毒素的浓度,羊水中前列腺素F_(2α)(PGF_(2α))和前列腺素E_2(PGE_2)浓度及血清孕酮的浓度。将子宫肌纵行纤维用1g等
To assess the effect of lipopolysaccharide on prostaglandin (PG) production and reactivity to oxytocin in pregnant rats on day 17 of gestation, indomethacin or saline was intraperitoneally injected to Wistar rats 17 days of gestation and one hour later, Polysaccharide or saline. The rats in group 2 (indomethacin group) were given indomethacin, then injected with normal saline, group 3 (lipopolysaccharide group) were injected with normal saline, Injected with lipopolysaccharide, group 4 (indomethacin - lipopolysaccharide group) first injection of indomethacin, and then injected lipopolysaccharide. Three hours later, the pregnant rats were anesthetized with ether, lavaged under asepsis, the blood was separated from the inferior vena cava and the uteri were removed. Each amniotic sac was isolated from the uterine horn and the amniotic fluid was collected. The concentrations of endotoxin in serum and amniotic fluid, The concentrations of prostaglandin F 2α (PGF 2α) and prostaglandin E 2 (PGE 2) and serum progesterone in amniotic fluid were measured. The uterine muscle longitudinal fibers with 1g and so on