论文部分内容阅读
以检测RFLPs进行各种药用植物种内或种间变异的分析,并把生药中残存基因的特定区域扩增克隆,根据检测其碱基序列的差异,来探讨用DNA鉴定生药的可能性。 1.三岛柴胡地理变异的分析:三岛柴胡(Bupleurum falcatum)在日本各地均有分布,为多态性强的种,由于产地不同其体细胞染色体数目、叶序、柴胡皂甙含量等形状和性质有差异,对日本8个产地野生的样品,在基因水平上进行了地理系统之间的遗传关系分析。首先选用御种植物,用RFLP图谱对个体变异进行探讨,但调查范围内同一种群未见个体变异。用各种限制酶进行RFLP的检测,在以Dra Ⅰ,Kpn Ⅰ,Taq Ⅰ进行限制酶酶切
The use of RFLPs for the analysis of intraspecific or interspecific variation of various medicinal plants and the amplification and cloning of specific regions of the surviving genes in crude drugs were used to explore the possibility of identifying crude drugs using DNA based on the differences in the base sequences detected. 1. Geographical variation analysis of Sandao Bupleurum: Bupleurum falcatum is distributed throughout Japan and is a highly polymorphic species. Its somatic cell chromosome number, phyllotaxis, and saikosaponin content are different due to origin. There are differences in shape and nature, and genetic relationships among geographical systems have been analyzed at the genetic level in wild samples from eight producing areas in Japan. Firstly, royal plants were selected and individual variation was explored using RFLP maps. However, no individual variation was observed in the same population within the investigated range. Detection of RFLP with various restriction enzymes, restriction enzyme digestion with Dra I, Kpn I, Taq I