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目的:探讨四妙勇安汤活性部位对一氧化氮合成酶抑制剂(L-NAME)诱导的高血压大鼠血管重构的作用及其机制。方法:48只Wistar大鼠随机分为6组,分别为四妙勇安汤活性部位高、中、低剂量组(1,0.5,0.25 g·kg-1·d-1),卡托普利组(50 mg·kg-1·d-1),辛伐他汀阳性药物组(10 mg·kg-1·d-1)及模型组,各组动物饮水中给予L-NAME(1 g·L-1)。给药第4周后,测定动物体重、心脏质量、血压、心率;处死后,主动脉根部切片苏木素-伊红(HE)染色,测定主动脉厚度变化;马松(Masson)染色观察冠状动脉纤维化变化;免疫组化方法观察单核巨噬细胞抗原-1(ED-1)和增殖细胞核抗原(PCNA)的表达变化。结果:与模型组比较,仅卡托普利组具有减轻心肌肥厚的作用;卡托普利组、四妙勇安汤活性部位高、中剂量组具有一定降低舒张压和收缩压的作用;与模型组比较,给药各组对心率均没有影响;卡托普利组、四妙勇安汤活性部位高、中剂量组可明显降低主动脉壁厚;卡托普利组、四妙勇安汤活性部位高剂量组可降低可明显减少冠状动脉周边纤维化的形成;卡托普利组、四妙勇安汤活性部位高剂量组可明显抑制冠状动脉ED-1和PCNA的表达(P<0.05,P<0.01)。结论:四妙勇安汤活性部位具有降低高血压作用,改善血管重构,其机制可能与其抑制冠状动脉ED-1的抗炎作用和抑制PCNA的抗增殖作用相关。
Objective: To investigate the effect and mechanism of Si Miao Yong An Tang active site on vascular remodeling induced by nitric oxide synthase inhibitor (L-NAME) in hypertensive rats. Methods: Forty-eight Wistar rats were randomly divided into 6 groups: high, medium and low dose group (1, 0.5, 0.25 g · kg-1 · d-1) (50 mg · kg-1 · d-1), simvastatin positive group (10 mg · kg-1 · d-1) and model group. L-NAME -1). After 4 weeks of administration, the body weight, heart mass, blood pressure and heart rate were measured. After sacrificed, the aortic root sections were stained with hematoxylin-eosin (HE) to measure the aortic thickness. Masson staining was used to observe the changes of coronary artery fibers (ED-1) and proliferating cell nuclear antigen (PCNA) were detected by immunohistochemistry. Results: Compared with model group, only captopril group had the effect of reducing cardiac hypertrophy; Captopril group and Simiao Yongan decoction group had the effect of reducing diastolic pressure and systolic pressure in high and middle dose groups; The model group, the administration of each group had no effect on heart rate; Captopril group, the active part of Simiao Yongan decoction can significantly reduce the aortic wall thickness; Captopril group, High-dose decoction of active fractions of the soup can reduce the formation of peripheral fibrosis of coronary artery obviously. High-dose captopril and active ingredients of Simiao Yongan decoction can significantly inhibit the expression of ED-1 and PCNA in coronary artery (P < 0.05, P <0.01). Conclusion: The active site of Simiao Yongan Decoction can reduce the blood pressure and improve the vascular remodeling. The mechanism may be related to the anti-inflammatory effect of inhibiting the coronary artery ED-1 and the antiproliferation of PCNA.