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目的 研究白细胞介素 -13 (IL -13 )对小鼠气道粘蛋白基因Muc5ac和表皮生长因子受体 (EGFR)表达的作用。方法 2 4只雄性BALB/c小鼠随机分为对照组和不同剂量IL -13 (包括 5 0ng、10 0ng和 2 5 0ng)处理组 ,每组 6只 ,分别用RT -PCR和免疫组化法检测Muc5acmRNA、Muc5ac和EGFR蛋白的表达。结果 Muc5ac和EGFR蛋白阳性表达细胞均主要位于气道上皮 ,细胞胞浆呈棕黄色。与对照组相比 ,IL -13可以剂量依赖性地促进气道Muc5acmRNA、Muc5ac和EGFR蛋白的表达 (P均 <0 0 1)。结论 IL-13是引起气道粘液过度分泌的重要细胞因子 ,它可能通过活化EGFR导致了上述病变。
Objective To investigate the effect of interleukin-13 (IL-13) on the expression of Muc5ac and epidermal growth factor receptor (EGFR) in mouse airway mucin. METHODS: Four male BALB / c mice were randomly divided into control group and different doses of IL-13 (including 50ng, 10ng and 250ng), with 6 mice in each group. RT-PCR and immunohistochemistry Method to detect Muc5ac mRNA, Muc5ac and EGFR protein expression. Results Both Muc5ac and EGFR positive cells were mainly located in the airway epithelium with brownish cytoplasm. Compared with the control group, IL-13 promoted the expression of Muc5acmRNA, Muc5ac and EGFR in airway (P <0.01) in a dose-dependent manner. Conclusion IL-13 is an important cytokine that induces airway mucus over-secretion, which may lead to the above-mentioned pathological changes by activating EGFR.