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目的探讨夏枯草水提物的抗抑郁作用及可能机制。方法小鼠分为正常组,模型组,盐酸氟西汀(阳性对照药,0.02 g·kg~(-1))组,夏枯草水提物低、中、高剂量(2,4,8 g·kg~(-1))组,给药组连续8 d灌胃给药。末次给药1 h后,采用小鼠悬尾、强迫游泳以及自主活动试验对小鼠进行行为学观察,并采用ELISA法测定小鼠海马组织5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)、脑源性神经营养因子(BDNF)、环氧合酶-2(COX-2)、前列腺素E_2(PGE_2)、白细胞介素-1β(IL~(-1)β)和白细胞介素-6(IL-6)的含量。结果各给药组与正常组比较,对小鼠自主活动无明显影响,差异无统计学意义(P>0.05);与模型组比较,夏枯草水提物中、高剂量组能显著缩短小鼠悬尾和强迫游泳不动时间(P<0.001),增加小鼠海马组织中5-HT、NE、DA的含量(P<0.05,P<0.01),降低小鼠海马组织中COX-2、PGE_2、IL~(-1)β、IL-6的含量(P<0.01,P<0.001)。结论夏枯草水提物可能通过提高海马组织中单胺类神经递质的含量、降低炎症因子的含量来产生抗抑郁作用。
Objective To investigate the antidepressant effect of Prunella vulgaris aqueous extract and its possible mechanism. Methods Mice were divided into normal group, model group, fluoxetine hydrochloride (positive control drug, 0.02 g · kg -1), low, medium and high dose (2, 4, 8 g · Kg ~ (-1)), and the administration group was administrated for 8 days continuously. One hour after the last administration, the mice were subjected to behavioral observation using mouse tail suspension, forced swimming, and spontaneous activity test. The levels of 5-hydroxytryptamine (5-HT), norepinephrine NE, BD, BDNF, COX-2, PGE 2, IL-1β ) And interleukin-6 (IL-6) content. Results Compared with the normal group, each administration group had no significant effect on autonomic activity in mice, the difference was not statistically significant (P> 0.05); Compared with the model group, the water extract of Prunella vulgaris medium and high dose group can significantly shorten the mice (P <0.001), increase the content of 5-HT, NE and DA in the hippocampus of mice (P <0.05, P <0.01), and decrease the expression of COX-2 and PGE_2 in the hippocampus , IL-1β and IL-6 (P <0.01, P <0.001). Conclusion Prunella vulgaris aqueous extract may increase the content of monoamine neurotransmitter in hippocampus and decrease the content of inflammatory cytokines to produce antidepressant effect.