论文部分内容阅读
目的探讨左归降糖舒心方对高脂饮食转基因2型糖尿病MKR小鼠糖脂代谢及炎症反应的影响。方法用左归降糖舒心方干预治疗高脂饮食转基因2型糖尿病MKR小鼠,采用电化学法测定空腹血糖浓度,采用全自动生化分析仪检测血脂和炎症因子超敏C反应蛋白(hs-CRP),采用ELISA法检测血清胰岛素与肿瘤坏死因子α(TNF-α)水平。结果高脂饲料喂养的MKR小鼠空腹血糖高于MKR组,但无统计学意义(P>0.05);血清胰岛素、血脂(TC、LDL-C、HDL-C)、血清hs-CRP和TNF-α水平高于MKR组(P<0.01),而三酰甘油(TG)下降,低于MKR组(P<0.01)。经左归降糖舒心方或文迪雅干预治疗后,除左归降糖舒心方低剂量组血糖和hs-CRP下降不显著(P>0.05),其他各组MKR小鼠血糖、血清胰岛素、TG、血清hs-CRP和TNF-α水平显著下降(P<0.05、0.01),TC和LDL-C下降无统计学意义(P>0.05);左归降糖舒心方高剂量组HDL-C显著上升(P<0.01),而文迪雅组升高不显著(P>0.05)。左归降糖舒心方作用呈剂量依赖性。结论左归降糖舒心方能调节高脂饮食MKR小鼠糖脂代谢、减少炎症因子的产生、保护血管、降低糖尿病并发血管病变的风险。
Objective To investigate the effect of Zuogong Jiangtangshuxin Recipe on glucose and lipid metabolism and inflammation in transgenic mice with type 2 diabetes mellitus (DM) with high-fat diet. Methods The Zuogui Jiang Tang Shuxin Fang intervention in high-fat diet transgenic type 2 diabetes mellitus MKR mice, fasting blood glucose concentrations were measured by electrochemical method, using automatic biochemical analyzer to detect serum lipids and inflammatory factor hypersensitive C-reactive protein (hs-CRP ), Serum insulin and tumor necrosis factor α (TNF-α) levels were measured by ELISA. Results The fasting blood glucose of MKR mice fed with high-fat diet was higher than that of MKR mice (P> 0.05). The levels of serum insulin, serum lipids (TC, LDL-C and HDL-C), serum hs-CRP and TNF- α level was higher than MKR group (P <0.01), while triglyceride (TG) was lower than MKR group (P <0.01). After the intervention of the left subsides Glucose-Shuxin-Fang or Wendia, the blood glucose and hs-CRP did not decrease significantly (P> 0.05) except the low-dose group of Zuogui Jiangtangshuxin and the other groups. The blood glucose, serum insulin, TG, serum levels of hs-CRP and TNF-α were significantly decreased (P <0.05, 0.01), TC and LDL-C had no significant difference (P> 0.05); HDL-C of Zuogui Jiangtangxin Fang high dose group (P <0.01), but no significant increase in Avandia group (P> 0.05). The effect of Zuogui Jiangtangshuxin was dose-dependent. Conclusion Zuogui Jiangtangshuxin can regulate glucose and lipid metabolism, reduce the production of inflammatory cytokines, protect blood vessels and reduce the risk of diabetic vascular disease in high-fat diet MKR mice.