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目的制备兔血清,用于兔来源细胞的培养。方法通过兔股动脉采血,4℃冷藏过夜后析出血清。分别比较含10%兔血清和10%胎牛血清的DMEM培养液中细胞形态和增殖性能及冻存存活率。结果使用兔血清培养细胞分裂指数约为1%~2%,冻存存活率为90%;使用胎牛血清细胞分裂指数约为0.5%;细胞冻存存活率为85%。结论兔血清制备方法简便易行,所获兔血清对兔来源的细胞在增殖性能和冻存存活率方面均优于胎牛血清,适宜兔源组织的培养。
Objective To prepare rabbit serum for culture of rabbit-derived cells. Methods Blood was collected from rabbit femoral artery and serum was precipitated after overnight at 4 ℃. The cell morphology, proliferation, and cryopreservation in DMEM containing 10% fetal bovine serum and 10% fetal bovine serum were compared. Results The cell division index of rabbit serum culture was about 1% ~ 2%, the survival rate of frozen storage was 90%, the cell division index of fetal bovine serum was about 0.5%, and the cell survival rate was 85%. Conclusion The rabbit serum preparation method is simple and easy. The rabbit serum obtained is better than the fetal bovine serum in proliferation and survival rate of rabbit-derived cells, which is suitable for the culture of rabbit’s tissue.