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目的：研究从菟丝子(Cuscuta chinensis Lam)中提取分离的酸性纯多糖CHC-1的结构特征及免疫活性.方法：利用化学方法和NMR光谱分析方法对CHC-1的结构特征进行了解析，并研究了CHC-1对小鼠抗体生成以及对淋巴细胞增殖率的影响.结果：CHC-1的分子量大于1.0×106.糖组份分析结果显示CHC-1是由Rha，Ara，Gal和GalA组成，比例为：0.8：1.0：1.5：0.3.甲基化结果和1H，13C NMR分析进一步确定了CHC-1中各残基的连接方式.CHC-10.1g/L能在体外明显升高T，B细胞的增殖率，CHC-1 25，50mg/kg显著增加小鼠的脾重，淋巴细胞的增殖率以及促进抗体生成.结论：CHC-1为一种多分支的杂多糖，并具有免疫增强活性.“,”AIM: To study chemical characterization and immunological activities of an acidic polysaccharide, CHC-l, isolated and purified from the seeds of Cuscuta chinensis Lam. METHODS: Both chemical and spectral methods were used to investigate the chemical characterization of CHC-1. Effect of CHC-1 on the proliferation rates of T- and B-lymphocytes both in vivo and in vitro, and antibody production in vivo was measured at various concentrations of CHC-1. RESULTS: The molecular weight of CHC-1 was estimated to be more than 1.0 × 106. The analytical results of sugar components indicated that CHC-1 was composed of Rha, Ara, Gal, and Gala in a molar ratio of 0.8: 1.0: 1.5:0.3. Methylation analysis and 1H, 13C NMR further identified the linkages of the residues of CHC-1. CHC-1 0.1 g/L promoted remarkably the proliferation of T-cells and B-cells in vitro. CHC-1 25 mg/kg, 50 mg/kg caused an evident increase in spleen weight, lymphocyte proliferation, antibody production, etc. But its effect on IgG levels was not significant. CONCLUSION: CHC-1 is a highly branched heteropolysaccharide and possessed immune enhancement activities.